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This is a combined synopsis/solicitation for commercial items prepared in accordance with FAR Part 12. This announcement constitutes the only solicitation; offers are being requested, and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q0963 is issued as a Request for Quotation (RFQ) for Genotyping Services.
This solicitation incorporates provisions and clauses by reference. The full text of provisions and clauses may be accessed electronically at www.acquisition.gov.
The applicable North American Industry Classification Standard Code is 541714 The small business size standard is 1,000 Employees This acquisition is a Total Small Business Set-Aside. All responsible sources may submit a quotation which will be considered by the agency.
See “Attachment 1 - Solicitation Terms and Conditions” **for applicable clauses, instructions, and evaluation criteria. **
The contractor must sequence 1824 DNAs extracted from brassica carinata leaf tissues (19 plates), including library preparation, library quality assessment, sequencing and genotyping using high throughput genotyping by sequencing technology, and bioinformatic data analysis and SNP calling. Sequencing must be completed using the Illumina NovaSeq X or similar platform with a read length of 2 x 150bp, and all samples must be run together in a single batch. The vendor must trim raw sequence data, analyze preprocessed data using TASSEL v5.0 GBS v2 pipeline or other pipeline, align to the Brassica carinata genome, and filter mapped SNP data converted to HAPMAP format. The guaranteed turnaround time from receipt of DNA samples is 60 days.
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