Acquisition of deep ultraviolet 304 nm laser for high-dimensional flow cytometry

Requirements:

• **TITLE **

Acquisition of deep ultraviolet 304 nm laser for high-dimensional flow cytometry

• BACKGROUND

The LP Research Flow Cytometry Facility provides essential cell sorting and flow cytometry analysis services to the Experimental Transplantation and Immunotherapy Branch, and other Branches and programs within the CCR.

This deep ultraviolet 304 nm laser is necessary for upgrade of our high dimension flow cytometers to allow excitation of deep ultraviolet fluorescent probes. This will allow expansion of our current high dimensional analysis capability without investing in an entirely new flow cytometer, since this unit can be installed on our Cytek spectral, LSRII, LSR Fortessa and Influx flow cytometers as required. It will allow both validation of these fluorochromes and their integration into current panels in the lab and support their use. Expansion of high-dimensional analysis capabilities requires these additional laser sources.

2.1 OBJECTIVE

The objective for this purchase is the acquisition of a deep ultraviolet 304 nm laser source suitable for flow cytometry. Deep ultraviolet 304 nm laser includes the laser unit, power supply, all required cabling and operations software. All items are included in the unit cost. Installation, optimization and verification is not included in the cost and will be carried out by NCI staff.

• SCOPE

The vendor will provide the system and supervise installation, as well as verification of operation. On year warranty support will be included with the system.

• CONTRACT REQUIREMENTS/ AND PERSONNEL QUALIFICATIONS

The contractor will provide technical support, both through telephone and e-mail. The local applications representative will be available both for routine support and also periodic on-site training.